Supplementary Information. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Softmax Pro GxP Software. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. personalized as your research. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. #W50-100). HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Both readers were heated to 37ºC prior to setting up . 100 fmol 1536-well (8 µL) 0. > Molecular Devices LLC > microplate reader. analysis software. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 0 fmol/well FITC 200 µL in 96 wells. M3-M5e_manual. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. It measures the optical density (OD) of samples in both 96- and 384-well. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. 2 Pages. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. 69: 85. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. Pick the best speed, sensitivity, and price for your needs. 96-well-plate-scan-time-2-colors-1-site-well. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Make sure to Data 96孔微孔板的数据对比. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. Please click on the links below to view the Microplate Reader Comparison Tables. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. Microplate Readers. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. Other Molecular Devices readers with absorbance detection mode, e. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The VersaMax and SpectraMax 190 read 96-well microplates. Endpoint, kinetic, spectrum. style. Brand: MOLECULAR DEVICES. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. No filter change with dual monochromator optics. 4. DNA concentration (ng/mL) Average RFU. Important things to consider when purchasing a multimode microplate reader. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. Paradigm: Delta mP. 1 sec. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Read one sample or up to 384 in a. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Description. , 384 wells < 45 sec. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Wavelength Range. We would like to show you a description here but the site won’t allow us. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. 03 pM 384-well: 0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Materials. Or scan up to 384 wells at any time to determine the best assay conditions. The device was able to achieve 3σ limits of detection of ca. #W50-100) SpectraMax® ABS Plus Microplate Reader. 2 2601A ValitaTiter. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. SpectraMax 250 User. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. Endpoint, kinetic, spectrum and area-well scanning Click here. 40266. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. validation, maintenance, and repair documentation. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. SOFTmax PRO allows you to select excitation and emission values from the literature and simply input their values for the monochromator settings. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Place the microplate in the drawer. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Compare this item. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. 0 96 Well Plate. Integrated stacker option. Plate reader instructions Computer. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 2 OD at 200 nm. LVF Monochromator™ : Excitation and Emission. 98 VMax R2 = 0. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. ZERO BIAS. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. , Sigma cat. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. M3-M5e_manual. Outer wells are aligned for proper viewing with FLIPR. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. 96-well microplate at 200 µL/well. Infinite 200 Pro Microplate Reader. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. About;. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. With various on-board features and capabilities, the ThermoMax is. 300 µL of Detergent Compatible Bradford reagent was added to each well. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Key applications: ELISA, nucleic acid, endotoxin testing. for ValitaTiter Assays. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. Four wash/rinse bottles are included and configurable for both 96- and 384. Molecular Devices has provided scientists with . What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Listing: #4772697. Optimized for fluorescence microplate readers. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. reagent basin for ease of pipetting into. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Microplate Readers. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. 2 W x 44. Table 1: Categories of heating element design. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 384-well: 0. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. A microplate reader as . The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 8 Pages. 00. Growth medium was then replaced with fatty acid uptake solution. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Total volume (except deep. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Avoid losing high signal. 05. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. ± 0. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 2 2600A ValitaTiter. #0310-5227) 96-well cell wash head (Molecular Devices Cat. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Both readers were heated to 37ºC prior to setting up the assay. • 0. The procedure enables direct translation of any published equation. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. of 0. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 7°C and the bottom to 36. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. 96, 384, 24, 12, 6 well types. Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. SmartReader 96: Designed for optimal ELISA Testing. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. or ValitaTiter Plus (Molecular Devices cat. $489. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Abstract and Figures. measurements in compatible Molecular Devices® microplate readers. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Plates can put in the drawer both in landscape and portrait positions. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Less background noise gives a better limit of detection and so a relative increase in usable signal. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 6:30 . 020 fmol 0. #T-0254) Lysozyme (Sigma cat. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Whatever your needs, you’ll find a microplate reader that can meet them. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 69: 85. 384 Awareness Technology ChroMate BioTek Synergy Mx. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. ” Promega Corp. Plate readers, also known as microplate readers or microplate photometers,. A multitude of modalities. Type: Microplate Reader. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. 4 Pages. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Supplier: Molecular Devices. 3% in white 96- and. Microplate is validated by molecular devices for the FLIPR reader. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. #N207) LAL Reagent Water (Lonza cat. 50 µL of. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. Omnia® Compatible Microplate Reader Documentation Version No. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Microplates . absorbance, fluorescence, and luminescence with the added functionality of. g. Other features and benefits include: Eliminate changing out filters. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. BMG LABTECH. and luminescence microplate reader validation DATA SHEET. 96- or 384-well microplate compatibility. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. Built in near field communication (NFC). The CellTiter-Glo assay from Promega uses luciferase enzyme. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. 3 OD at 200 nm. Align the two slots on the bottle holder with the two pegs on the rail. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. The SpectraMax® ABS Plus Microplate Reader can run . Elisa Plate Readers and Microplate Readers at Biocompare. 05. 1536-well: 0. Top or bottom detection is available with a simple click of a button. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Description. High tolerance for contaminants. Flexible for 6-well to 384-well microplates and cuvettes. Molecular Devices i3x. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Samples were run in triplicate. This page contains the. VANTAstar™ Flexible Multi-mode Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Cross-talk < 0. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. A. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 2022 Harini Natarajan et al. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. U-bottom: 30-40 µl. We would like to show you a description here but the site won’t allow us. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. book Page 9 Wednesday, July 7, 2010 2:09 PM. You can add your own protocols. 63. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. flexibility of monochromator-based systems. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. i3x. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 9. Reconstitution of. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. This . The VersaMax reads 96-well microplates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Industry standard, 96-well plates are. Running both standard spectrophotometer and microplate. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Jon Cuccui, Brendan W. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 54: 1. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Category 2 and 3 microplate readers use gradient heating as well. Setup Guide. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. 3. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. San Jose, California, June 5, 2018. Plate Formats: 6 to 384-well microplates. Molecular Devices VersaMax Microplate Reader Specifications. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 03 pM. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Model:. Three low volume 24-well bottom slides. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. For biochemical. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Protein quantitation Cross-talk < 0. Keywords: fluorescence polarization, trf, fret, igg. Five-second read per plate. Keywords: fluorescence polarization, trf, fret, igg. A preconfigured.